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This volume describes numerous applications of sponge-matrix culture to study cancer and the treatment of cancer, stem cells, growth and repair of nerves, lymphoid tissues that produce antibodies, and the HIV infection. The chapters in this book cover topics such as in vivo-like growth patterns of multiple types of tumors in Gelfoam® histoculture; development of histoculture drug response assay (HDRA), hair-shaft growth in Gelfoam® histoculture of skin as well as isolated hair follicles in Gelfoam® histoculture; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam® histoculture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, 3D Sponge-Matrix Histoculture: Methods and Protocols is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture."
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