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Genetic Engineering Strategies
Alter levels of Biochemicals Demonstration through Vitamin B5 pathway Adapted for NextGen Bioinformatics era
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Genetic or Metabolic Pathway Engineering capabilities and knowledge enables standard lab resources and public databases information to be used for wonders by manipulating molecular levels. The techniques discussed in this article are those generic to any biochemical pathways concerning cellular or sub-cellular environment that can play pivotal role based on its characteristics. Demonstrated step-by-step and logically deduced using pantothenate committed pathway as example pathway which poses magnitudes of complexity one can face while designing experiments. The methods are to up-regulate or down-regulate the metabolic flux of a junction or to channelize over/under production of metabolite, to quantify the effect using efficient & inexpensive bioassay techniques and statistical models. The book works as a guide to those aspiring to learn the art of how research work is carried out in low budget environment. For those willing to deepen their understanding on metabolic flux control for better understanding of genomic loci function, the strategies and techniques discussed are indispensable for commercial gains. The book can be used as study reference in University senior level.
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