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Protein Design: Method and Applications, Second Edition expands upon the previous editions with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements, characterization of fluorinated proteins, symmetric protein folds in an approach, functional sites of proteins, and computational design of novel enzymes without cofactor. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.§§Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical protocols and reviews to aid in the creation of new experiments.§
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